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From measured microclimatic data and comfort surveys, carried out in downtown Curitiba, Brazil, the impact of street geometry on ambient temperatures and on daytime pedestrian comfort levels was evaluated, using the sky-view factor (SVF) as indicator of the complexity of the urban geometry.
The statistical significance of the ECT2 expression levels was evaluated using Fisher's exact test or Mann-Whitney U test.
Numerical quantification of changes in expression levels was evaluated using the DataSyngene program.
The statistical significance of the differences between average evolutionary conservation levels was evaluated using the Students' t-test.
IFNγ production at the single-cell levels was evaluated using intracellular cytokine staining from PBMCs following stimulation as indicated [ 24].
Given the nonparametric distribution of hsCRP levels, relationship between age and hsCRP levels was evaluated using the Spearman rank correlation test.
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5mC and 5hmC global levels were evaluated using the ELISA-based MethylFlash Methylated DNA Quantification Colorimetric Kit (P1034, Epigentek) and the MethylFlash Hydroxymethylated DNA Quantification Colorimetric Kit (P1036, Epigentek) respectively.
Finally, the effects of plant scale factors on impact levels were evaluated using grey model GM (1, N) too.
Intracellular calcium levels were evaluated using a FURA-2/wire myograph system.
Intra-group differences in plasma FDG and glucose levels were evaluated using the Student's t test.
Gene expression of FGF2 and VEGFA on the mRNA and protein levels were evaluated using quantitative polymerase chain reaction and enzyme-linked immunosorbent assay methods.
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