Sentence examples for levels was calculated using from inspiring English sources

U6 snRNA was used as an internal control, and the relative amount of miRNA normalized to the U6 snRNA levels was calculated using the 2-ΔΔCT formula, in which ΔΔCT = (CT miRNA - CT U6) target - (CT miRNA - CT U6) control.

For mouse and human samples, percent change in mRNA levels was calculated using the formulas ((Experimental group/PBS group) ×100)) – 100% and ((Glioma/Normal brain) ×100)) – 100%, respectively.

The correlation analysis between clinical features and peak intensity levels was calculated using bivariate correlation analysis.

Relative quantification of mRNA levels was calculated using the comparative CT method.

The correlation between TDI and Mn urinary levels was calculated using SPSS software (version 17).

Statistical significance of the treated versus untreated IL-8 levels was calculated using a t-test with Welch's correction.

Relative expression levels were calculated using 2−△△Ct method2−△△Ct

Expression levels were calculated using the relative quantification method.

The relative expression levels were calculated using the 2−ΔΔCt method2−ΔΔCt

mRNA levels were calculated using the LightCycler 480 software.

Relative expression levels were calculated using CFX Manager 3.1 (Bio-Rad) software and the ΔΔCT method23.