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The dosage effect of LT on the suppression of cell viability and intact MEK1 levels was analyzed using J774A.1 cells (Fig. 1A, B), a mouse macrophage cell line commonly applied for the study of LT function [13], [18].

Damage as a function of treatment (3 levels) was analyzed using a generalized linear model with mixed effects, utilizing the glmmPQL command in the MASS package (R 2.8.1, R Foundation for Statistical Computing, 2008).

Correlation of protein expression levels was analyzed using Spearman rank correlation analysis.

Correlation between the pathologic variables and CXCL1 levels was analyzed using Chi-square test.

Relative quantification of specific mRNA levels was analyzed using the cycle threshold (Ct) 2-ΔΔCt method.

The association between IA-2βA units and IA-2A levels was analyzed using nonparametric Spearman correlation.

Glucose and lactate levels were analyzed using the YSI 2700 select analyzer.

Gene expression levels were analyzed using Cufflinks.

Cellular cAMP levels were analyzed using the cAMP-Screen direct chemiluminescent competitive immunoassay system (Thermo Fisher Scientific), according to the instructions of the supplier.

Chimera or negative control chimera were incubated with HT-29 or HEK-2913T cells for 48 hours and the survivin protein levels were analyzed using Western blot analysis.

Plasma GABA levels were analyzed using high-pressure liquid chromatography (HPLC) with fluorescence detection after derivatization with o-phthaldialdehyde (OPA).