Sentence examples for levels specific activity from inspiring English sources
When the enzyme activity of the expressing Tg-S466L mice was normalized for CBS tetramer levels, specific activity of p.S466L was again found to be almost equivalent (0.53 ± 0.23 µmol/mg/hr) to wild-type hCBS in the presence of AdoMet (data not shown).
Level specific activities and an opportunity for reflection must exist hand in hand with role modeling.
A typical trade-off occurred between soluble expression levels and specific activity for all single mutants, but many double mutants decreased both properties more severely suggesting a critical loss of protein stability or native folding.
Cathepsin B protein levels or specific activity did not differ between cells in the presence or absence of CysC (data not shown).
When considering the quality of recombinant GLA enzyme, the primary concern is its ability to be purified while retaining relevant levels of specific activity.
The results of activity screening (where activity can generally be considered to be the product of both expression levels and specific activity) at each round are summarized in Table 1.
When the enzyme activity of the Tg-S466L mice was normalized for CBS protein levels, the specific activity of p.S466L was found to be almost equivalent (0.6 ± 0.26 µmol/mg/hr) to wild-type CBS in the presence of AdoMet.
This was not an unexpected result considering that T. reesei is a known producer of high quality recombinant enzymes; however it was significantly more challenging to reproduce similar levels of specific activity from ZERA-GLA produced intracellularly.
A glycosomal glycerol kinase (Tb09.211.3540) related to human CARKL, which is required for caveolin/lipid raft-mediated endocytosis [ 18], was upregulated in PCF, although equivalent levels of specific activity for this enzyme have been reported for both life stages [ 80, 87].
Performing whole cell biotransformation experiments, the enzymatic hydrolysis of pNO2PheDU (4a) could be shown with the highest level of specific activity after 2 h with 0.326 mU/mgcdw and a total conversion yield of 72.27 % after 24 h.
The labelling of BoNT-A to a level of specific activity necessary for sensitive detection by PET, i.e. 1 kBq/ng BoNT-A, was accomplished by this IODOGEN-coated BoNT-A method in a yield of 51%to53%3%.
