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These results suggest that Dhh1 is shifting the balance of Pab1 from the translating pool to the non-translating pool, likely by either promoting the accumulation of Pab1 into aggregates containing both proteins and mRNA, or by heightening the levels of translation repression, thereby shifting the mRNA into non-translating pools and subsequently triggering stress granule formation.
Transfection and staining of S2 cells with these FLAG-tagged smORFs confirmed the translation of all 12 smORFs tested, which exhibit a range of translational indicators and peptidomics evidence, indicating that even lower levels of translation can give rise to smORF peptides detectable by this tagging method.
Regulation of protein synthesis by viruses occurs at all levels of translation.
We show that promoterless fusions have value for determining levels of translation, whereas fusions to inducible promoters have utility in the analysis of mRNA-binding factors.
Using polysome analysis and RNA-binding assays, we show that elevated levels of translation depend on an interaction between a triple arginine motif in LC3 and the AU-rich element in Fn1 mRNA.
Without dipping too far into interieur base-ball, there are several levels of translation via computer: machine translation, done solely by the computer, at the sentence level; machine-assisted translation, in which a human translator cleans up a translation done by a computer, and translation memory, stored phrases and sentences, for boilerplate paragraphs in documents.
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Taken together, the results suggest that control at the level of translation, translational selection, is the dominant mechanism underlying differential protein expression in these organisms.
Our data also agree with previous studies in that we observed increased lifespan after reducing transcript levels of translation-related genes.
The low correlation between DNA microarray and proteomics data [47] has also implicated extensive molecular control at the level of translation and post-translational modification.
Translational control mechanisms, particularly acting at the level of translation initiation, can be a primary point of regulation for certain genes.
They too bind proteins (creating microRNPs), but control gene expression as the level of translation and mRNA stability in the cytoplasm.
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