The transcript levels of the PSR responsive genes AtPT2, AtACP5, and AT4 increased in response to Pi deficiency.
Under abiotic stresses, ABA is often recruited as the primary signal for increasing the transcription levels of the stress responsive genes.
Except for the expression of ACO2 and ETR2 in the leaves, the transcript levels of the ethylene responsive genes decreased compared to 24 h of exposure to Cd.
Furthermore, application of Phi in Pi sufficient or deficient conditions to tir1- 1 did not affect the transcript levels of the auxin responsive genes tested with the exception of AXR1 in 0.5 mM Phi-treated, Pi deficient plants.
In the tir1- 1 mutant, Pi starvation did not considerably change the transcription levels of the auxin responsive genes AUX1, AXR1, AXR2 and SGT1B confirming a role for TIR1 in induction of the PSR.
Moreover, Pi starvation did not considerably change the transcription levels of the auxin responsive gene AUX1, AXR1, AXR2 and SGT1B in the aba2- 4 and tir1- 1 mutants confirming a role for ABA2 and TIR1 in the induction of the PSR.
(A) to (E) Quantitative Real-time PCR analysis of mRNA levels of the glucose-responsive genes APL3, BAM, GBSS1, GPT2, PDC1 in Col and pft1-2 in response to glucose.
As previously described, the levels of the ACTH-responsive aldose reductase-like AKR1B7 followed the same pattern, confirming the efficiency of the hormonal treatments [31].
In line with this, overexpression of AtGRP7 entails reduced levels of the JA-responsive PDF1.2a but does not prevent its induction by MeJ.
To analyze whether there is a perturbation of ecdysone accumulation in these animals, we measured the transcript levels of the ecdysone-responsive gene E74B as a proxy for the ecdysone titer during the larval stage.
