The different levels of the five selected factors were shown in Additional file 1: Table S1.
Then the central levels of the five significant factors identified from PBD are determined by steepest ascent design.
The expression levels of the five genes were higher in OPAO line #11 than in fgl mutant, indicating that leaf recovery in the OPAO lines is closely associated with an increase in the expression levels of these genes (Fig. 6).
So, the transcription levels of the five genes (pou5f1, cdx2, igf2, igf2r, and hdac2) whose functions are mentioned above were detected by quantitive PCR (Fig. 5) at the blastocyst stage.
In this study, the best levels of the five independent variables that provide the maximum modified resolution for each pair of consecutive amino acids appearing in the chromatograph were determined.
In a mechanically stirred tank reactor and in the presence of pure nitrogen gas, statistically designed experiments were performed at two levels of the five operating parameters which were the free cyanide concentration, temperature, cementation time, base and precious metal concentrations and their studied ranges were 50 150 ppm, 60 90 °C, 15 90 min, 175 1350 ppm and 220 880 ppm, respectively.
Transcript levels of the five qRT-PCR-confirmed genes in aCRSwNP were relatively distinct when compared to the AR samples (Figure 2).
The RNA levels of the five genes were distinct during tail metamorphosis versus the metamorphic intestine (compare Figure 2 and Figure 4).
We found that there was no significant difference in the levels of the five snRNAs in 15.5d embryos and postnatal day 5 mouse tissues between the retina and all other tissues (Fig. 4).
The mRNA levels of each gene were normalized relative to the mean levels of the five housekeeping genes and compared with the data obtained from unstimulated, serum-starved cells using the 2−ΔΔ Ct method.
Differences in the expression levels of the five genes leads to extreme SI values.
