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(D) Correlation of p27 phosphorylation levels at two different sites (T157 and T198) with mRNA expression levels of targets in 750 breast cancer patient samples.
We examined the consequence of tamoxifen treatment on muscular transcript and protein levels of targets selected for their known contribution to CNM pathogenesis, for modulating Mtm1−/y mouse phenotype, or mediating tamoxifen actions (Fig. 4; Supplementary Tables 4, 5).
We quantified mRNA and protein levels of targets involved in inflammation and anti-viral activity (INFβ1, IL-8, RANTES/CCL5, IFIT2, IFIT3, ST3GAL4, ST6GAL1, IL-10), localized sialic acid receptors, and assessed mitochondrial function.
Relative expression levels of targets were calculated by the comparative cycle threshold (Ct) method outlined in user bulletin no. 2 provided by Applied Biosystems.
These in vitro findings are consistent with the modified levels of targets we observe in the wing, namely Dll, nmo, Sens and Ac.
Expression levels of targets analyzed were calculated relative to GAPDH expression, using the ΔΔ Ct method.
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(C) Protein level correlation of p27 with mRNA levels of target genes in 750 breast cancer patients.
Right: Tolerability of lanicemine vs ketamine, measured by hyper-locomotor activity, at comparable levels of target engagement.
The relative expression levels of target genes were compared to those of β-actin (mouse) and GAPDH (human).
(G) Expression levels of target genes in combination in less-invasive and invasive cancer cell lines from NCI60 panel.
Relative mRNA levels of target genes were normalised to Gapdh and Actb gene expression and related to the AL-control.
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