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Relative levels of target mRNA expression were calculated using the 2-ΔΔCT method.
The relative expression levels of target genes were calculated using the 2−ΔΔCq method.
In a comprehensive detection attack, an adversary wins by achieving all the three levels of target given in Definition 5.9.
However, for both housekeeping genes, the relative levels of target gene expression presented almost the same pattern of changes.
So the presence of low levels of target DNA can potentially result in a significant enhancement of ECL signal.
Expression levels of target genes were normalized to 18s RNA.
The levels of target gene expression were quantified relative to the level of GAPDH, using the standard curve method.
To normalize the expression levels of target miRNA, the U6 small nuclear RNA was used as a control (housekeeping).
A real-time quantitative polymerase chain reaction was used to quantify the mRNA expression levels of target genes.
The GAPDH gene was used as an internal control to normalize the expression levels of target mRNA.
Three days later the initial levels of target cell transduction were determined by eGFP-directed flow cytometry.
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