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The octree is then refined to avoid discontinuities between the levels of subdivision of two adjacent octants.
Nevertheless, users must carefully specify the initial mesh and/or painstakingly manipulate the control vertices at different levels of subdivision hierarchy to satisfy a diverse set of functional requirements and aesthetic criteria in the limit shape.
Higher levels of subdivision were necessary for accurate inference as subpopulation number increased, possibly due to fewer individuals per subpopulation or to bias in the Fst and G'st estimators.
In contrast, Southeast Alaskan populations show much greater levels of subdivision.
These unexpected levels of subdivision are unlikely to have arisen under gene flow, so these four newly-detected groups can be seen as good evidence for the existence of cryptic species previously unrecognized by morphological techniques.
For example, levels of subdivision among populations of four species of fish, six bivalves, three gastropods, and one urchin, in this region were all less than in A. tenuis (Johnson et al. 1993).
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Serrano et al.[ 8] have highlighted the influence of animal exchanges in the Spanish Guadarrama goat breed, which explain the high level of subdivision observed with microsatellite analyses.
We performed additional Structure analyses with K = 3, which could potentially match the level of subdivision in PUUV (note that K = 3 had a lower likelihood than K = 2).
Accuracy levels for datasets with many markers highly subdivided by population may be high largely because of structure; we therefore calculated R m 2 – – excluding markers with high levels of population subdivision as indicated by their Fst values, where high Fst indicates high population subdivision (for methods, see File S1).
The degree of population genetic subdivision in C. sorrah in northern Australia in this study was similar to the overall low levels of genetic subdivision reported in a previous allozyme study [data not shown, [ 51]].
In what follows we describe the application of PCO-MC to four exemplar data sets with varying levels of genetic subdivision, from highly structured to virtually unstructured.
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