Fluorescence minus one (FMO) controls were used to determine background levels of staining.
Isotype-matched control antibodies were used to determine background levels of staining.
Immunofluorescence staining of Dnase1l1 showed low levels of staining with comparable intensity in kidneys from mice of all ages (data not shown).
The pyramidal neurons in the cornu ammonis (CA) and the polymorphic layer of the dentate gyrus exhibited particularly high levels of staining that contrast with the lack of staining in adjacent areas such as the molecular layer (Figure 2A).
There were low levels of staining on macrophages, cultured in either SFM or with serum and M-CSF, with both prolyl-4-hydroxylase and collagen-I antibodies (Figures 1, 8 and 10), indicating that macrophages may produce collagen, as described previously [34], [38].
The majority of outer root sheath (ORS) follicular keratinocytes co-expressed C/EBPα and C/EBPβ in their nuclei as did the vast majority of sebocytes of the sebaceous gland which displayed the highest levels of staining intensity for C/EBPα and C/EBPβ (Fig. 1B).
In addition, levels of staining for MMP-13 did not correlate with levels of staining for TIMP-1.
Isotype-matched fluorescently-labelled control antibodies were used to determine background levels of staining.
Cutoff levels reflect levels of staining not observed in normal colonic mucosa controls.
A set of dsRED-transfected cultures was stained with secondary antibody alone in order to determine background levels of staining.
However, tenocytes exhibited an absence or very low levels of staining for these cell markers, further confirming that TSCs and tenocytes are two different types of tendon cells.
