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Seeds are capable of accumulating high levels of seed storage proteins (SSP), as well as heterologous proteins under certain conditions.
Treatments consisted of four levels of seed treatment with molybdenum and biofertilizers (Rhizobium + PSB) and four levels of foliar spray of boron, laid out in split plot design.
Vegetative reproduction enables small patches to persist for many years and apparently maintains genetic diversity despite low levels of seed production.
Contrary to expectations, the probability of setting fruits and seeds as well as the levels of seed predation were similar among trees growing across the three contrasting landscape conditions.
First, loss of GL2 activity results in higher levels of seed oil, deficiency in seed mucilage production, collapsed columella, light seed color and slightly reduced PA deposition3,22,23,24 (Supplementary Fig. 16), which are similar to those observed in ttg1 and tt2 mutants9,15,17,38,40 (Fig. 3a and Supplementary Fig. 10).
Our data suggest that the consequences of habitat fragmentation for genetic differentiation and genetic diversity of A. vulneraria are relatively minor which is very likely due to the historical high levels of seed exchange among fragments through grazing and roaming livestock.
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Our initial experiments expose that budgets planned based on (G') in networks with heavily skewed degree distributions (as in many real-data examples) can lead to wasteful levels of seeding.
Larger promoter fragments significantly increased levels of seed-specific gene expression.
Promoter analysis revealed that although cis-elements extending 1470 bp upstream of the transcription start site can modulate gene expression, the proximal 295 bp is sufficient to drive high levels of seed-specific GUS activity.
Field observations did indicate very low levels of pod- and seed-set over the species range despite high levels of seed-set in many other sympatric species in the year of sampling (M. Millar, DPAW, Perth, Australia, unpubl. res).
Levels of seeds that remained ungerminated in the late fall was also significantly affected by % crop alleles, but represented the inverse pattern seen for germination for this removal (data not shown).
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