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Expression levels of probe sets were calculated using GCRMA, followed by identification of differentially expressed probe sets using Limma.
The E. coli pBAD/btuE strain and the complemented ΔbtuE mutants showed decreased levels of probe activation relative to non-overproducing strains (Table 2).
Levels of probe intensity suggested that this sample gained one X chromosome.
However, we were unable to directly measure low levels of probe remaining bound to the bead.
Expression levels of probe sets were normalized by GCRMA [ 34], using M-estimators for summarization.
We expect that the higher the quality of a given array, the more consistent the levels of probe signal from the same probe set.
Similar(47)
Basically, it takes as input a gene expression dataset and compares, in each sample, the expression levels of probe-sets that are reliable indicators for activity of a given miRNA seed "miR-seed", with the expression levels of other probe-sets present on the array, which serve as reference.
The existence of population-specific differences in expression levels of probes was determined by comparing the expression data using a Kruskal-Wallis test.
As this multidimensional scaling plot showed a wider dispersion of group-1 WTs, a Bartlett test was performed to compare levels of probe-specific variance between group-1 and group-2 WTs.
We tested the expression levels of probes that overlapped with endo-siRNA reads.
This table shows prime analyses at levels of probes and genes for normal models and swap models.
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CEO of Professional Science Editing for Scientists @ prosciediting.com