Inhibition of CXCL12 (1 ug/ml AMD3100) and FGF signalling (500 nM PD173074) had no effect on the levels of co-localisation (results not shown).
Co-localisation was quantified using a mask generated from the streptavidin channel as explained in Figure 2 figure supplement 3. Importantly, as the different markers label abundant structures it was possible that low levels of co-localisation would be detected due to chance overlap rather than specific labelling of the same structures.
This means of regulation of EpCAM effects at the level of subcellular localisation was further underscored by experiments using conditional systems in vitro.
Regulation of the TRAIL receptors, at the level of expression, localisation to the cell surface and the O-glycosylation of the proteins, partially, but not fully correlates with sensitivity [ 9- 11].
Our culture density data suggests that some scFvs are more toxic than others and that clones with a high level of supernatant localisation hinder bacterial growth following induction, perhaps through lytic mechanisms or destabilisation of the outer membrane.
Immunostaining showed higher level of MLL4 localisation primarily in the cell nuclei of the control xenograft tissues, while in the MLL4 antisense-treated xenograft, MLL4 was localised in patches of fragmented nuclei and the level of expression of the protein was relatively less compared with the untreated control.
Here, we go beyond the summary statistics and explore image level variability, precision of localisation responses and the potential influence of guesses to test whether detection is possible without localisation.
To validate our mass spectrometry data and ascertain high levels of cell surface localisation of EPHA2 on OS cells and low levels on hp-OBs, cells were subjected to FACS analysis.
We show that PP1γ is indeed subject to a striking alteration in both its levels of expression and localisation, both as lesions develop, and in the tumour derived cell lines.
The levels of expression and localisation of CYPs 1A1, 1B1 and 2E1 in malignant and adjacent normal colorectal tissue were determined using a modified avidin/biotin immunohistochemistry procedure (Goggi et al, 1986).
