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We hypothesized that global methylation levels in cancer, represented by levels of LINE and SINE (Alu) methylation, might be correlated with viral infection.
For instance, the CHG methylation levels of line PNA 1.2 indicated only 13.1% (±7.1) in seedlings but 90.9% (±4.0%) in flowering plants.
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Lower levels of LINE-1 methylation were associated with lower EDSS scores in MS patients, while Alu showed higher level of methylation in the group of patients with low EDSS score [46].
The levels of LINE-1 demethylation may therefore be helpful in several clinical situations.
First, we evaluated the accuracy of AQAMA in assessing various levels of LINE-1 methylation.
The levels of LINE-1 transcripts in DicerCKO PGCs were almost half of those detected in control PGCs (Figure 3B).
Interestingly, CIMP+/MSI-and CIMP-/MSI-showed higher levels of LINE-1 hypomethylation, reinforcing the uniqueness of CIMP+/MSI+tumors.
We investigated five normal colon mucosa and five peripheral blood lymphocyte (PBL) DNA samples from healthy donors to determine the normal levels of LINE-1 methylation.
In addition, we found that male cases displayed significantly higher levels of LINE-1 methylation than female cases (Pearson's correlation −0.466, p<0.001), and cases with higher age at surgery displayed lower levels of LINE-1 methylation than younger cases (Pearson's correlation 0.320, p = 0.018).
There has recently been reported a significant inverse correlation between levels of LINE-1 methylation and the total number of chromosomal aberrations, including both losses and gains in gastrointestinal stromal tumors (GIST) [7].
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