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However, to our knowledge, no one has attempted to directly select for freezing differences following fear extinction after matching animals for post-acquisition levels of freezing.
We show that CBD-treated animals had increased levels of freezing during conditioning, enhanced generalized fear, inhibited cue-dependent memory extinction, slightly increased levels of freezing during an auditory-cued memory test, and increased contextual fear memory.
After conditioning, all odors evoked similar levels of freezing behavior, regardless of similarity to the threat-predictive odor.
Levels of freezing during the pre-tone period were comparable between mutant and control mice (F1,15 = 2.28, P = 0.15).
Comparable levels of freezing between control and RU-486-control mice indicated that treatment of RU-486 itself exerted little effect on the fear conditioning.
During the conditioning period, SCR mice showed lower levels of freezing after foot shocks [Fig. 1A, Conditioning, p = 0.0276, f(1,38) = 5.246].
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During the training (Figure 6C, left), no significant effect of the genotype was observed on the level of freezing (two-way ANOVA, F 1,22) = 1.54; p = 0.23), and all mice reacted alike to the US (F 1,22) = 89.37; p<0.0001).
As anticipated, the level of freezing tolerance was positively correlated with the period of time under acclimating conditions.
The high level of freezing seen in the naïve animals is indicative of good learning and memory retention.
No correlation could be established, for example, between variations in the proline pool during cold exposure and the level of freezing tolerance for Arabidopsis and Thellungiella sp. [ 32].
The existence of positive correlations between the level of freezing tolerance of cereals and their capacities to accumulate water-soluble carbohydrates including sucrose and glucose was also previously observed [ 21, 35- 37].
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