Absolute levels of differentiation were also higher in cells differentiated from 2i.
Several analyses were performed by successively removing the most differentiated populations to reveal all levels of differentiation among populations.
Differentiated cells possess more and longer neurites and express higher levels of differentiation markers (GAP43, RET).
But even within the subcategories, there are levels of differentiation.
The results obtained showed that the methods tested in the study gave different levels of differentiation between the strains tested.
Pairwise Fst estimates based on candidate directional selection loci also demonstrated increased levels of differentiation between study populations.
Significantly higher levels of differentiation markers, namely, alkaline phosphatase and glycosaminoglycans, were detected in the scaffold cultures.
Sub-population 5A had moderate but similar levels of differentiation as group 1 (FST of 0.36) group 2 (0.34) and group 6 (0.31).
The expression levels of differentiation markers except Flk1 in the chip with a diameter of 1000 μm were similar to those in hanging drop culture.
Using the method of phenotypic evaluation of progenitor cells of various levels of differentiation in the tumor focus makes it possible the identifying the stages, dynamics of development and invasiveness of the process.
For instance, the computation of levels of differentiation (Fst) indicates that craniometric traits behave much as neutral markers: around 10 15% of variation is accumulated in differences among populations (Relethford 2001 , 2002.
