Exact(1)
Dilutions (1 20) of these pre-amplifications were used as templates for amplification with primers designed for the kinase-2 motif of the NBS region of plant disease resistance genes, with varying levels of degeneracy, combined with primers designed to one of the adapters with varying numbers of selective bases (one, two or three).
Similar(59)
A procedure for performing Monte Carlo calculations of plasmas with an arbitrary level of degeneracy is outlined.
Since the occurrence of triple bonds is rather infrequent, we contend that this low level of degeneracy is much more acceptable than the drastic and unwanted increase in component count.
This high level of degeneracy provides virtually unlimited coding potential to ensure both developmental buffering and flexibility to deal with random external factors.
The discovery of these multiple ways of controlling the excitability reveals there is a substantial level of degeneracy behind the action potential response.
The high level of degeneracy precluded identification of relationships from multiple sequence alignments; we thus, used a size-based criterion to identify subfamily relationships.
ARS identification in S. cerevisiae by genome-wide motif scanning has been hampered by the abundance of sequences with high similarity to the ACS, combined with the level of degeneracy of the ACS that supports function.
The well-established high level of degeneracy of binding of these allergen peptides to a range of HLA class II molecules further supports the ability to manufacture a population-based therapy, rather than requiring detailed patient endotyping and individualized medicines.
As nucleotide sequence identities of the corresponding cDNAs in these regions were very high, degenerate primers were designed based on nucleotide sequences in these regions keeping a low level of degeneracy (Additional file 4: Table S2).
We attribute this to a higher level of degeneracy within this higher-order repeat family in vivo, with the cloned fragment being divergent from the most common sequence predicted by the genome survey.
In contrast to only 64 codon combinations in the genetic code, the number of possible 'ciphers' in the epigenetic code or the level of degeneracy could be many orders of magnitude higher [ 53].
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