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One subject had to be excluded due to high levels of artefact in the anatomical scan.
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More studies with different stains, or specific studies without staining (freezing techniques), would be needed to determine the level of artefact within this protocol and hence the physiological relevance.
We previously published an informatic pipeline to assess the level of artefacts in archival lung squamous cell carcinoma samples [ 12].
Significantly, changes were inversely correlated with coverage indicating high levels of sequencing artefacts were associated with samples with low amounts of available amplifiable template due to fragmentation.
Further digging revealed a second layer of artefacts.
The clinical evaluation showed that PET/MR offers higher image quality and sharpness with lower levels of noise and artefacts compared to PET/CT with reduced acquisition times for whole body scans, while for brain scans there is no significant difference.
Arrays were quality checked manually, removing spots affected by high levels of background or artefacts.
Importantly, the elevated levels of VHR are not an artefact of cell culture.
Differential levels of importance placed on cognitive artefacts, particularly patient-authored Birth Plans, which were either not acknowledged or used ineffectively in some cases, further undermined the positive effects of handover described by participants.
However, higher levels of cytoplasmic accumulation are likely an artefact of the overexpression of exogenous Dystrophin.
This is true for both PrP overexpressing mice and for wild-type mice, supporting that PERK-eIF2α overactivation is not an artefact of high levels of protein expression, but occurs at endogenous levels (consistent with observations in humans).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com