Exact(2)
Different levels of anchoring efficiency, ranging from 20 to 60%, were observed [ 34], demonstrating that human GPI-T can prioritize amongst different substrates by recognizing subtle differences in signal sequences.
Each type of discrimination was measured in two conditions (XAB and XXXAB) designed to afford different levels of anchoring by varying the number of repetitions of a standard stimulus (X) prior to the presentation of the test tone (A or B) in each trial.
Similar(58)
This observation suggests that GPI-T participates in governing the cell surface concentration of substrate proteins by promoting different levels of anchor attachment.
We addressed these deficiencies and achieved a high level of anchoring first, by utilizing the resequencing data and the SNP calling pipeline, combined with an "inverse mapping" strategy of planned genetic map construction.
Each layer represents a broader span of considerations and an increasing level of anchor point complexity over that below it.
The advantages of anchoring proteins via GPI anchors vary depending on the protein anchored and the organism concerned [ 8].
Two human GPI-T signal sequences were also tested and each showed diminished extracellular INV activity, consistent with lower levels of GPI anchoring and species specificity.
For example, early work demonstrated that expression of the Trypanosoma brucei VSG (variant surface glycoprotein) in COS cells led to protein expression but only low levels of GPI anchor attachment; this defect was rescued when the VSG C-terminal GPI-T signal sequence was replaced with the human decay accelerating factor signal sequence [ 36].
In contrast, the use of a human GPI-T signal sequence significantly reduced the level of GPI anchoring [ 37].
When the anchors' coordinates are known up to a confidence level, they can be modeled as ordinary sensors, connected to an imaginary anchor (with infinite mass and set at the believed location) by a hard spring, with strength k much higher than others, reflecting the confidence level of the anchor in its coordinates.
After the participants' voices were recorded reading the Japanese RB vs. LB ambiguous phrases, we randomly paired participants with a stranger5 and used the TANDEM-STRAIGHT toolbox to morph their original voices into four transitional levels of similarity using manually anchored start and end points of each syllable.
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