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In Figure 4E, we showed that expression levels of analyzed factors remain unaltered post-AHSCT in MSCs and monocytes.
Quantitated mRNA levels of analyzed genes were normalized to GAPDH mRNA to generate a relative expression ratio.
The expression of each ATG under control conditions was appointed as 1 at each time point, and the levels of analyzed mRNA were normalized according to the relative GAPDH mRNA expression of each sample.
Transcript levels of analyzed cytokine transcripts were higher in the former group than in the latter and the differences reached statistical significance (p ≤ 0.05) by Mann Whitney test for IL-8, IL-16, TNF-α, GM-CSF, MCP-2, TGF-β (Table 2).
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The table shows the variation in the concentration level of analyzed parameters during wet and dry seasons.
The standard cycling conditions were 95°C for 10 min, followed by 40 cycles of 95°C 15 s and 60°C for 1 min. The comparative Ct method (2-ΔΔCt method) was used to determine the expression level of analyzed genes [64].
Level of analyzed markers expression was established on the basis of 12-point IRS scale (immunoreactive score) by Remmele [ 6].
The comparative CT method (2-ΔΔCT method) was used to determine the expression level of analyzed genes [ 90].
On the level of analyzed partial ORFs, 1 SNP was found in 45 partial ORFs, 2 SNPs in 25 partial ORFs, 3 SNPs in 4 partial ORFs, and 4 SNPs in 1 partial ORF.
The mRNA relative amount was estimated from the CT value according to our previous description (Jiang et al. 2007) and was used to evaluate expression level of analyzed genes.
Summary of expression levels of KATs analyzed in this study normalized to the expression of GAPDH.
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