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A mixture of 18 TDCs (dioxins, dibenzofurans and PCBs) was tested at doses comparable to human exposure levels for effects on serum T4 in rats.
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The equivalent human body burden for the no observable adverse effect level for effects of TCDD in the most sensitive rats on bone growth, modeling, and mechanical strength is estimated to be approximately 11 ng/kg body burden (Jamsa et al. 2001).
Although the bottlenose dolphin dose response data include both natural and contaminant-mediated effects, the mortality is still less than the threshold level for effects in pinnipeds reported by Ross et al. (2000) and Kannan et al. (2000) of between 17 and 20 mg/kg.
Although the results of our study are therefore not directly comparable with this value (representing a guide level rather than any absolute threshold) because the total PCBs were estimated using slightly different methods, it provides an indication of an approximate threshold level for effects determined by others.
One level of each categorical factor was set as the reference level for effect assessment.
The covariates were included in the models as interaction terms with the binary exposure variable [< 50 vs. ≥ 50 dB(A)], using a Wald test to assess statistical significance of overall interaction (α level for effect modification = 0.10).
This critical U-Cd level for glomerular effects was lower and closer to the critical levels for tubular effects than expected from previous studies.
An a priori power analysis with a minimum cell size of N = 3, a minimum detectable difference of two n-back levels for main effects (hypotheses 1 and 2), and one level for interaction effects (hypothesis 3) revealed a power of >.80 to detect main effects and of >.60 to detect within-subjects interactions (PASS software, NCSS Inc., Kaysville, Utah).
Thus far, the critical UCd levels for kidney effects have displayed large variations.
Threshold levels for mercury effects were tested at quintile cutoffs and at the 90th percentile.
More studies need to be done to establish risk levels for adverse effects.
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