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Glycerol level was determined using Free Glycerol determination kit according to the manufacturer's instruction (Sigma, USA).
Total NCL level was determined as input.
Then, the serum malondialdehyde level was determined.
Serum leptin level was determined by radioimmunoassay.
Fasting blood glucose level was determined at 7 days interval.
d LoNA was pulled down by anti-NCL antibody, level was determined by qPCR.
(f) H3K9me2 level was determined by western blot using Odyssey CLs.
Supernatant was decanted and left at 4 °C until rAChE level was determined.
Serum cholesterol level was determined by a cholesterol quantitation kit (Sigma-Aldrich).
In those cases, the 0% (or 100%) level was determined using the smaller (or larger) averaged value.
c NCL was pulled down by LoNA DNA antisense probes by RIP, level was determined by western blot.
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