The level of translated protein from 756 expression constructs was measured and the influence of a large number of possible effector attributes was statistically analyzed.
In the tissues of CAG200 mice, the level of translated EGFP protein was significantly reduced, whereas the transcript level was not affected by the repeat sequence (Fig. 2B D).
Using bacterial artificial chromosome-translating ribosome affinity purification (BAC-TRAP) followed by microarray analysis, they compare levels of translating mRNAs in cortical astrocytes (specifically, Aldh1L1-positive cells in cortex and striatum) and find 1.4%% of astrocyte transcripts increase or decrease with sleep compared with transcript levels during wakefulness and sleep deprivation.
Impaired mRNA decay can lead to accumulation of transcript with a consequent effect on levels of translated protein (2).
To elucidate whether TACE mRNA overexpression in human HNSCC results in corresponding levels of translated TACE protein, we conducted immunoblot analysis using an antibody specifically recognising the carboxyterminal part of human TACE.
Besides identifying morphological changes, examination of bronchial tissue can provide information on mRNA expression and subsequent levels of translated inflammatory mediators directly within the tissue, both by resident cells and infiltrating inflammatory cells using immunohistochemistry (IHC) [ 10- 12].
It was focused on the advantage itself at the level of firms capable of translating this advantage into a product or service introduced in different markets.
Though in both E and MT life cycle stages the transcriptome and the translatome are well correlated (Pearson correlation coefficient r = 0.78 and 0.66 respectively, Additional file 6), we considered that translatome data, which represent the levels of translating RNAs, should be a better approximation of protein levels than transcriptomic data.
In a top down analog system design methodology, the task of translating high-level performance specifications and constraints into component level parameters is termed as constraint transformation.
Functional studies aim to understand how changes in the DNA level of variation translate to the (regulation of) RNA transcript levels, protein levels and metabolite levels.
