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The effect of beclomethasone on the level of transcript was validated by quantitative reverse-transcription polymerase chain reaction (qRT PCR).
Transcription levels are expressed as the ratio to the level of transcript at 0 h in WT.
When studying the subgroups the TGF-β -EMT module, the integrin-matrix-EMT module and the transcription factor module distinguished IPF from control primarily at the level of transcript abundance.
The relatively low gene expression levels reported here likely reflects a low reverse transcription efficiency of the primers but may also reflect a low level of transcript expression.
Using a calibration algorithm improved the accuracy of the array in quantifying the relative level of transcript expression.
The two alleles of SUB1A have a single amino acid difference and are distinct in the level of transcript induction during submergence.
The level of transcript in midgut was higher at night, but the levels in ovary and female accessory reproductive gland showed the opposite pattern.
Conversely, unique genes had a diminished level of transcript variance.
Furthermore, 7.2% of isoform changes occurred at the level of transcript initiation or transcript termination.
This is because the value indicates the minimum required level of transcript for viability, not the minimum inhibition level of transcript, and hence differs from IC50 in this respect.
Interestingly, only segmental duplicate genes had an altered level of transcript variance in response to the treatment variation (Figure 2).
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