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Brenner and colleagues [ 1], in their article published in Critical Care, showed elevated levels of the reactive carbonyl species (RCS) methylglyoxal (MG) in the circulation of patients with septic shock.
Changes in the intracellular levels of the reactive oxygen species (ROS) were analyzed by the fluorescent dye 2',7'-dichlorodihydro fluorescein diacetate (DCDHF-DA, Molecular probes, Life Technologies Corporation) as it has been already described by Stankova et al. 2013.
Data presented in Figure 3(a) clearly demonstrates that SiHa cells display lower levels of the reactive oxygen species detected by both DCFDA and DHE than do CaSki cells.
The level of this reactive metabolite may be correlated with the species differences in butadiene sensitivity.
Antioxidants reduce the level of reactive oxygen species and reactive nitrogen species generated by oxidative stress in response to acoustic trauma, aminoglycoside and platinum-based drugs.
OS is defined as a state in which the level of toxic reactive oxygen intermediates overcomes the endogenous antioxidant defenses.
The level of intracellular reactive oxygen species (ROS) was determined by the change of fluorescent probe dichlorofluorescein diacetate (DCFH-DA).
Serum-starvation increased the level of intracellular reactive oxygen species, and this was reduced by the addition of CX3CL1.
E2 also increased the level of intracellular reactive oxygen species (ROS) in MCF-7 cells in the absence of radiation.
The level of thiobarbituric reactive substances (TBARS) was assessed in tissue homogenates (10% w/v prepared from frozen samples).
This machinery minimizes the level of cytotoxic reactive oxygen species (ROS) that can inflict irreversible macromolecular and cellular damage.
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