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Some seasonal variations in the level of selected pesticides were noted.
Furthermore, we analyze the expression level of selected cell adhesion-related genes by the quantitative real-time (qRT-PCR) method and the cell viability for MWCNT powders.
The transcription level of selected candidate genes was verified by RT-PCR using GAPDH as the control.
First, the mRNA level of selected genes was quantified using real time RT-PCR (qRT-PCR) (Figure S5).
A detailed examination at protein and functional level of selected targets of TCF/LEF transcription (e.g. MMP2, MMP14 and TIMP3) [26] confirmed these observations.
mRNA level of selected genes was confirmed by qRT-PCR.
By comparison to β-actin (internal control), the expression level of selected genes was determined.
The expression level of selected miRNAs was monitored by quantitative real-time PCR.
Quantitative PCR (qPCR) was performed to determine the expression level of selected unigenes.
RNU44 and RNU6B were used for normalizing the expression level of selected miRNAs.
Therefore, we have made an attempt to assess the level of selected indicators at different stages of the disease (rDD).
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