Sentence examples for level of probe from inspiring English sources

A greater level of probe mismatching is tolerated within a microarray hybridisation (1 6 base differences over 60-mer probe can reduce signal intensity by at least 50% (LeProust [2008]; Hughes et al. [2001])) compared with lower levels of mismatch that are permissible within a quantitative PCR experimental design.

Contrarily, in the PM-G'G duplexes saturation is much less relevant owing to the smaller average level of probe occupancy and intensity.

Cells (105), suspended in 750 μL of ABB or medium without serum, were first preloaded with NBD-PS by adding 2 μL (for HeLa, macrophages and lymphocytes) or 10μL (for fibroblasts) of a 30 μM stock of NBD-PS (prepared as described above) followed by incubation for 3 min, to minimize the level of probe internalized by nonspecific endocytosis (macrophages) or 10 min (lymphocytes, Hela and fibroblasts).

This approach assumes a linear relationship between age and log-2 expression level: <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0013098.e001.PNG" class= inline-graphic"/> Here Yij is the log-2 gene expression level of probe set i in sample j, Aj is the age for individual j, Sj is 0 if individual j is female, 1 if he is male.

The most frequently used calibration method, retrodialysis, was used to assess the level of probe recovery.

Only perfect match (PM) probes were included in the estimation for expression level of probe sets.

We took into account the success and survival, peri-implant crestal bone loss, and the level of probing at which the gum bled.

The appellate judges subsequently requested three rounds of post-oral argument supplemental briefing, a rare level of probing that indicates to many observers that the court is likely ready to reverse Minor's conviction.

The average expression level of probes in chimpanzee and human was calculated using data obtained from normalised chimpanzee and testis microarray data described above.

To calculate the average expression level of probes representing autosomal and X-linked genes in different chimpanzee and human tissues, probe id's were annotated with chromosome number and gene name (Affymetrix HG-U133 Plus2 Annotations, release 30).

To better understand the relationship between hybridization success rate and sequence conservation we examined the level of probe-target mismatches in available sorghum sequences.