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The level of pERK is higher in CFA and IS treated groups.
SKAP-55−/− T-cells showed a 2 4 fold greater level of pERK staining from 1 10 minutes.
Co-immunoprecipitation experiments also revealed that CN-A bound to PERK and that the phosphorylated level of PERK was significantly higher after 30 minutes of OGD (Figure 6B).
Further, retroviral transduction of primary SKAP-55−/− T-cells restored the level of pERK staining to wild-type levels (data not shown).
The same stimulation of Z.21, Z.22 and Z.23 cells showed a two to five fold higher level of pERK (lanes 2 4 versus lanes 8 10; 14–15; 20–22).
One of the most common biomarkers used is the level of pERK tumour biopsies.
However, surprisingly, at high levels of FGF2, addition of heparin was predicted to increase the level of pERK response.
Indeed, the low threshold level of pERK in these cells may promote their sensitisation to MEK inhibition.
In contrast, IGF-1 elicited a strong response only at the level of pAKT and FGF-1 at the level of pERK.
The effects of adhesion on the level of pERK in MDA-MB-435 and MDA-MB-231 cells contrasted those of pMEK.
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