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Each of these can also be upgraded to a second level of lethality.
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Coexpressing UAS.dSno and UAS.MamN generates a low level of lethality with 89.2% of expected adults observed (235 experimental compared to 292 siblings).
All the strains tested showed increased lethality in comparison to E. coli OP50 baseline, but the Classical strains caused a lower level of lethality than the El Tor strains (Fig. 4A).
We thus expect that any secondary effects linked to the level of lethality are minimal in our experiments.
Cep290 gt were backcrossed onto 129SvJ and Rachel et al. reported a significant level of lethality, as was reported for the Cep290 KO model, with the vast majority of Cep290 gt mice dying in utero between E12 and E14.
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Ubiquitous RNAi using Act5C-Gal4 or Da-Gal4 resulted in severe levels of lethality.
These conditions of heat shock caused similar levels of lethality (5 8%) in control and Mnn1 mutants (Supporting Information Table S1).
BTV infection of IFNAR −/− mice via this route induces similar levels of lethality, viraemia and pathology to those generated via an intravenous route (Javier Ortego, personal communication).
Previous data showed that some H9N2 isolates from domestic poultry possessed the ability to replicate in mice without prior adaptation, and cause varying levels of lethality [8], [12], [13].
Once the challenge infection of IFNAR −/− mice with AHSV-4 had been characterised and that doses in the order of 106 pfu/mouse induced clinical signs, viraemia and significant levels of lethality, we evaluated the protective efficacy of a recombinant MVA expressing VP2 of AHSV-4 (MVA-VP2).
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