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Perreault, W. E., Mukherjee, N. & Zare, R. N. Preparation of a selected high vibrational energy level of isolated molecules.
Extensive atomic force microscopy (AFM) studies at the single pore level of isolated, fixed nuclei reveal changes in the NPC conformational state following treatments that stimulate Ry receptor activity.
Data from our study support the notion of a direct cardiac depressant effect of lethal toxin at the level of isolated cardiomyocytes.
These data confirm the impaired beta cell function in P2X7-KO mice from our in vivo studies on the level of isolated islets.
However, many proteins are part of oligomeric complexes and analysing protein stability on the level of isolated chains does not capture the physiologically relevant situation in the cell.
These results clearly indicate that at the level of isolated AKO endothelial cells the major characteristics of endothelial dysfunction are no longer evident.
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This effect is not unexpected considering that in the limiting case of an "infinitely" dilute monolayer one ought to arrive at the "isolated molecule" situation and the energy levels of isolated donor- and acceptor-substituted molecules clearly differ from each other.
We chose random RT-PCR for template cDNA preparation, because of the low levels of isolated RNA from the specimens.
Remarkably, in MG132-treated cells ubiquitination levels of isolated eEF1A1-HIS wild-type and corresponding mutants were comparable.
As shown in Figure 4 ubiquitination levels of both isolated phosphorylation resistant mutants of eEF1A-HIS (S21A and T88A) were increased in comparison with the ubiquitination levels of isolated phosphorylation-mimicking mutants (S21D and T88D) in MG132 non-treated cells.
The levels of isolated AGP were determined spectrophotometrically. Preparation of the glycans was similar to methods reported by Fan et al. [ 17].
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