Sentence examples for level of inducer from inspiring English sources
(b) Post-induction temperature and post-induction period at fixed level of inducer concentration.
In contrast, the qa-2 system was unable to achieve expression above native levels at the greatest level of inducer, and these cultures had a WT period (Cheng et al. 2001).
Our result is reproducibly seen in three different strain backgrounds, and with different levels of inducer (data not shown), so currently we have no explanation for this discrepancy.
It has been shown that when an externally repressed circuit is induced, an overshoot of transcriptional response is observed in response to steady levels of inducer, which then falls back to its pre-induction level (due to pumping out of the inducer by multi-drug efflux pumps), rendering the cell resistant to further induction [64].
No deleterious effects on cell growth were observed due to the high levels of inducer.
In contrast, the HTD circuit is designed to express low levels of tetR at low levels of inducer and high levels of tetR at high levels of L-arabinose (see Figure 11(C)).
We tested varying levels of inducer ranging from 0 to 1000 µM arabinose and observed no growth impact compared to the control at any concentration of inducer (Fig. 2d).
This approach allows us to measure both the cost and benefit of lac operon expression for independently varying levels of inducer and carbon source, and to directly determine the optimum expression level of the metabolic genes for each carbon source concentration.
We consider it unlikely that these differences are due to differences in expression levels from these plasmids, since the promoter sequences are identical for the BF- nanO and BF- nanOU constructs and there was no difference in levels of inducer added (i.e. none).
In fact, the apparent acetate repression might be accounted for by acetate metabolism via acetyl CoA and effects on uptake of compounds and intracellular levels of inducers.
The high levels and cellular patterns of CYP1A1 expression are similar to those seen in animal models exposed to high levels of inducers; other mammals do not show this broad pattern of induction unless exposed to high concentrations of contaminants.
