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The level of incorporated radioactivity, which corresponds to the phosphorylation status, was quantified by Scanalyze.
Interestingly, DegS S76D maintained a considerable level of incorporated phosphate in the presence of DegU, whereas DegS wild type and DegS S76A retained much less phosphate under these conditions.
The level of incorporated H-thymidine was assessed using Beckman liquid scintillation counter.
Proliferation was assessed according to the level of incorporated tritiated thymidine.
The level of incorporated BrdU was measured at OD370 with an ELISA reader (TECAN).
Similar(54)
Lifetimes of luminescent levels of incorporated holmium and ytterbium ions and the Yb3+ − Ho3+ energy transfer efficiency were determined based on analysis of recorded luminescence decay curves.
Mortars with IOT showed increased bulk density, reduced levels of incorporated air, an increment in the amount of mixing water, and improved mechanical properties when compared with conventional ones.
The levels of incorporated DHA achieved in our study are similar to previous reports in both murine [30], [31], guinea pig [12] and human cells [41], which reported DHA levels exceeding 10 mol% in vivo following fish oil feeding.
2) While the authors show that the liposomes made with or without PE, DAG and ergosterol have similar levels of incorporated SNARE proteins, the authors should provide more evidence that these two populations of liposomes are really comparable.
The effects of salmon calcitonin on human articular cartilage proteoglycan synthesis were investigated in response to different doses (100 pM-100 nM). Figure 1, shows the proteoglycan synthesis under the different conditions as determined by the levels of incorporated SO4.
Of note, in both cell cultures, the presence of immature VLPs raised significantly with the cell passages (not shown) In conclusion, our data indicate that, upon induction, 18-4s cells produce HIV-1 VLPs whose morphology was not influenced by the high levels of incorporated NefG3C-GFP molecules.
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