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The level of immobilization score ranged from 1 (no immobilizing or sedative effect) to 6 (excessive immobilization depth with respiration <3 breaths/min).
This difference is likely due to different methods of immobilizing the ssDNA MRE as noted above and thus leads to a lower level of immobilization and detection responses.
A linear part of this dependence was observed in the range from 0 to 0.5 μM SPA-Cys, whereas 2 μM SPA-Cys corresponds to the close-to-saturation level of immobilization [26].
The level of immobilization of the rhinoceros also did not differ significantly between different trials.
A more superficial level of immobilization eliminated some of the drug induced depression on circulation and breathing reflexes.
After the desired level of immobilization was achieved, a 1 M ethanolamine solution was injected to deactivate remaining active esters.
Similar(50)
The levels of immobilization were 120 RU and 138 RU for IGF-I and -II, respectively.
Final levels of immobilization were approximately 500 response units.
Higher levels of Fab immobilization resulted in non-saturable binding suggestive of non-specific binding and thus were not used.
The C1 chip did not support levels of HSP72 immobilization that allowed detection of binding by the small peptide analytes.
Lower levels of HSP72 immobilization (i.e., ligand RU) were not used because peptide concentrations similar to the KD produced poor signal under these conditions, thereby limiting the range of peptide concentrations that could be analyzed.
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