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Owners can also list their favorite local restaurants and coffee houses and describe their level of housekeeping standards.
While the level of housekeeping σD was similar in parent and mutant, the levels of the flagella synthesis regulator σF and the stationary phase regulator σS were higher in the mutant strain, indicating a different motility and stationary phase phenotype.
Not even a five star hotel can achieve that level of housekeeping; they may tidy your room three times a day, but that's plenty of time to trash it in between.
The results were normalized to the mRNA level of housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Hs99999905, Applied Biosystems, Switzerland) and expressed as relative values compared to the control group.
Autophagy is a protein degradation process by which cells recycle cytoplasmic contents under stress conditions or during senescence; a basal level of housekeeping autophagy also occurs under non-stressed conditions.
The expression level of housekeeping gene ABL was also determined as the control.
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We correlated the median expression levels of housekeeping genes25 across all non-tumor samples and found a strikingly high degree of agreement between the datasets (Pearson R = 0.973, p-value < 1 × 10−20) (Fig. 1c; Supplementary Fig. 1).
Levels of "housekeeping" proteins are maintained directly, but those of immune response proteins depend on more transcription.
To further assess the robustness of the single cell data, the levels of housekeeping genes were examined.
The expression levels of housekeeping genes across eight tissues were higher compared to tissue-specific genes (Figure 2).
We adjusted this baseline difference by estimating the difference between the expression levels of housekeeping genes common to the two datasets.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com