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The number of epistatic interactions for each level of flux restriction is included in Additional file 10: Table S5.
If the level of flux control at a given step changes based on environmental condition, this would cause a significant genotype by environment interaction.
If the relationship between fitness and flux is linear near the optimum level of flux, then deleterious mutations will play a reduced role.
Furthermore, the internal xylose catabolism pathway used in conjunction with heterologous or engineered xylose transporters can determine the level of flux control exhibited by the transport step [ 67].
In the upper regions of the pathway, the level of flux after the introduction of a statin-like inhibitor is well below that achieved in immune-led responses.
We assumed a level of flux into the pathway ∼2/3 rds of the lowest Vmax value obtained across both time courses.
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Simulations show that even at Crab levels of flux, the Con-X microcalorimeter does not "latch" into the normal state.
This concept implies that if threshold levels of flux are exceeded, compensatory mechanisms may fail, leading to inadequate development.
For instance, sorting for different levels of induced flux can be used to isolate cells that are either efficient inducers of flux in response to a given stimulus or poor inducers based on their levels of flux.
Proteins may have different levels of flux control under different growth conditions; their flux control can even be in opposite directions depending on the nature of the competition.
However, this type of hierarchical control does not take place in general since there are several levels of flux regulation which operate separately from the purely genetic.
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