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CVs for high and low level of external standard and for in-house plasma pool were 5%, 7.9%and3.5%5% for triglycerides and 5%, 7.6% and 3.8% for glucose.
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Quantification was performed on the basis of external standard method.
Quantitative analyses were performed by means of external standard methods.
The results were absolutely quantified by comparison to the external standards curves (see section Generation of external standard curves).
Data quality was monitored by the T2 determination of external standards in each set of images.
Products were identified by retention times of external standards chromatographed at the beginning of the experiment.
A requirement of 70 80% of cancer cells seems reasonable in order to compare telomerase levels to external standards provided by cell lines (Marchetti et al, 2002).
Hence, the results have a high level of external validity.
On each trial, random external pixel noise was chosen from a Gaussian distribution, with one of eight levels of external noise contrast with standard deviations 0, 0.04, 0.08, 0.12, 0.16, 0.25, 0.25, or 0.33.
A series of 10-fold dilutions of an external standard was used in each run to produce a standard curve.
Consequently, the use of an external standard curve is acceptable for the quantification of 8-oxodG.
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