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When extending the analyses to all 566 SNPs in the respective gene locus, this level of correction may be too permissive.
Typically, as the level of correction for multiple comparisons increases, the line between apparent heterogeneity and homogeneity is increasingly blurred.
This level of correction and expected somatic mosaicism may be sufficient in some but not all cases.
A 10-20% level of correction was achieved with VX-809 in a recent published study [ 55].
Three different approaches were used to calculate the FRET efficiency of analyzed cells that introduce increasing level of correction for sample-induced artifacts.
It is now felt that a 50% level of correction is a better benchmark that is equivalent to 27°C reduced temperature correction in a biochemical correction assay.
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I found that the public papers have a seven to eight-fold higher level of corrections and retractions.
These introduce increasing level of corrections for sample induced artifacts, stemming from the decrease of GFP fluorescence lifetime with increasing expression level.
Some evidence of plasticity of, for example, bone marrow cells, has been demonstrated, although for the airway, levels of correction appear to be extremely low and a degree of damage seems to be a prerequisite.
EBs transduced with this vector showed similar levels of correction of disease phenotype as revealed by NBT assay (Figures 5A and 4A).
The "same rate slower" and "slower same rate" contrasts produced no significant differences at whole-brain levels of correction.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com