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As shown in Figure 8, transfection with an increasing level of CIS expression plasmid results in increased ERK reporter activity.
The level of CIS mRNA reached a maximum at four hours and remained high for at least 18 hours.
Various enzymes, such as amylase, UDP glucose pyrophosphorylase or invertase, have been suggested to control the level of CIS in tubers either by increased or suppressed expression or activity [ 8- 10].
Prolactin receptor mRNA was expressed in all but one of the transformed lines and not in the two control lines, but the level of prolactin receptor expression did not correlate with the level of CIS mRNA across these lines.
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In i), activation level of cis-acting sites is discretized (beyond on/off) based on transcription factor concentration (see above).
Because of the importance of Gene Regulatory Networks in the generation of organismal complexity [1], the assessment of the level of cis-regulatory sequence activity and protein function during metazoan evolution can be particularly insightful [2], [3].
We follow Buchler et al (2003) to integrate combinatorial signal at the level of cis-regulatory transcription control.
However, they do not allow us to systematically determine the extent of regulatory network conservation at the level of cis-regulatory mechanism.
In general, the level of cis-aconitic acid and glyceric acid is higher, while the level of glutamine, asparagine, proline, trehalose and unidentified m/ z 186 is lower in shoots than in crowns.
Based on the genome-wide DNA methylation sites, we calculated the absolute methylation level of cis-NAT genes, trans-NAT genes and non-NAT genes at CG, CHG and CHH sites, respectively.
Taken together, our results indicate that human and fruit fly TF binding specificities display a striking level of conservation, despite dramatic morphological differences resulting from more than 600 million years of evolution and lack of detectable sequence conservation at the level of cis-regulatory elements.
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