The level of amplification is depended on sediment thickness.
One of the primer pairs and the corresponding probe displayed a high level of amplification efficiency on which a real-time RT-PCR method was established.
Because the DSM is not rigidly fixed to the Port Plug structure, the DSM and its internal components suffer, during abnormal events like seismic or electromagnetic disruptions, an important level of amplification on the accelerations induced.
Although I found the level of amplification nearing my pain threshold, there's no denying that the audience had a rowdy, good time.
The level of amplification of cyclin A2 and A1 were too low to precisely measure variation in expression.
The level of amplification is presented as a percentage of the internal standard EF1alpha gene (that contains no HpaII site), to normalize genomic DNA variations between samples.
The fact that tumours with different copy numbers of a given gene are available offered us the possibility to determine the relationships linking the level of amplification to that of mRNA expression.
Nevertheless, the results obtained with tumours arboring different copy numbers of the EGFR, SEC61G, LANCL2 and VOOP1 genes allow us to establish that mRNA over-expression parallels the level of amplification (Figure 1C).
Whilst a level of amplification is not as high as typically described in neuroblastoma [35], we find significant over-expression of N-myc target genes in C5 tumours supporting the view it is functionally active.
The results of qPCR were analyzed in the same way: the binary coding for qPCR reactions was based on whether or not mouthwash-extracted DNA samples achieved a threshold level of amplification (the threshold level having been chosen to approximate the minimum amplification level sufficient for successful microsatellite genotyping).
HER2 presented the highest level of amplification.
