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Also on protein level, HOSE cultures showed negative to negligible levels of EpCAM expression (Bellone et al, 2009).
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But jargon-slingers' vocabulary hieroglyphics often limit everyone's bandwidth when the troops really just want to drink from a high-level fire hose while the cement is still wet and the competition is still in the weeds.
The hose level, first described in 1629, consisted of a length of hose fitted with a glass tube at each end.
A notable finding from this comparison is that whereas we identified the miR-200 family of miRNAs (miR-200a/b/c, miR-141 and miR-429) to be expressed at low levels in normal HOSE with highly increased expression in ovarian cancer, the study using E6/E7-immortalized HOSE did not find increased expression of miR-200 family miRNAs in ovarian cancer.
AT a gas station the other day, I saw an advertisement attached at eye level near the fuel hose.
The water level is simply a hose with short lengths of transparent plastic tube at each end.
Of course automated systems like a hose pipe level with a connected data logger might measure data in shorter time periods.
Western blotting confirmed low CCBE1 expression in low GFP-expressing cells and high CCBE1 expression in the GFP high cell population, which was comparable with the level of CCBE1 in HOSE 6.3 cells.
This unexpected low DNA methylation level for EpCAM in HOSE cells is in line with data of differentiating human embryonic stem cells, where epcam silencing was not associated with increased DNA methylation (Lu et al, 2010).
All epithelial ovarian cancers, regardless of their histology, overexpressed SCGB2A1, CLDN3, CLDN4, TROP-2 and SCGB1D2 mRNA at significantly higher levels when compared with HOSE (P<0.001).
Besides the evaluation of protein expression, we also determined kallikrein 6 mRNA levels between healthy human HOSE primary cell cultures and borderline and invasive tumours.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com