No mutations were observed among 34 glioblastomas or 89 leukemias tested.
Nevertheless, if ALL samples with these gene fusions were not included into the analysis, PDE4A was significantly upregulated in TTLshort leukemia (T-test, N=16, TTLshort n=6, TTLlong n=10, P=0.024).
In summary, we have devised a protocol to expand GDTc to clinically relevant numbers, developed a bioluminescent xenogeneic model of Ph+ leukemia and tested GDTc therapy in this model.
In two of the four leukemia lines tested, no drug resistance emerged after repeated drug treatment, and this correlates with the clinical course of the patients in question as these individuals remain in clinical remission.
Because the variant ARID5B rs10821936 allele was remarkably associated with an increased risk of MLL-r acute leukemia, we tested whether this risk allele was associated to a specific MLL TPG or to any of the frequent MLL breakpoint regions.
Association between the candidate SNPs and the susceptibility to childhood acute lymphoblastic leukemia was tested using gender adjusted logistic regression in the case control data performed by IBM SPSS Statistics software (version 19.0).
Quinacrine was the only compound found with activity in the three leukemia subtypes tested with concurrent low toxicity in normal mononuclear cells, and was, therefore, selected for further preclinical evaluation.
To probe which, if any, of the studied cell line models is relevant for human acute myeloid leukemia, we tested leukemic blasts isolated from patients with diagnosed APL or AML (see Supplementary Table S1 for details of disease).
The copy number of 1p36.33 and mtDNA peripheral blood mononuclear cells infected by the Epstein Barr virus in patients with lymphocytic leukemia was tested in quantitative PCR by Jeon et al. They suggested that increased mitochondrial biogenesis is indicative of the progression of EBV-mediated B-cell transformation [ 13].
