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The two extreme cases are columns that are highly conserved where the most prevalent letter receives a large positive score and all others large negative ones and columns that are highly variable and close to neutral where all letters receive scores close to zero.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com