This loss of interaction is relevant in vivo since we found that pol3-ct confers HU sensitivity on its own and synthetic lethality with a POL32 deletion.
The mutation results in early embryonic lethality with a subpenetrant phenotype of kidney agenesis.
Targeted disruption of murine Cdk2ap1, an inhibitor of CDK2 function and hence G1/S transition, results in the embryonic lethality with a high penetration rate.
We have shown here that Hira-mutants in young males lead to a higher paternal-effect embryonic lethality with a phenotype of chromatin bridges.
Here, either through a random screen or starting from an educated guess, we have looked for mutations causing synthetic lethality with a thermosensitive allele of RPS15 to find genetic interactors potentially involved in nuclear export.
It is a disease of insidious progression and high lethality, with a 5 year survival rate of only 6%.
Consistent with facilitating replication fork restart, smc-5 mutants exhibit synthetic lethality with a mutation in the DNA primase div-1 that functions in DNA replication.
Depletion of tyms-1 and npp-6 resulted in embryonic lethality with a penetrance of 89% and 99%, respectively (Table 2).
Ms5Yah mice display high postnatal lethality, with a few surviving individuals showing growth retardation, motor coordination deficits, and spatial learning and memory impairments.
TBPH mis-expression had broadly deleterious effects, causing early lethality with a variety of different cell-type specific drivers (data not shown).
Consistent with previous observations (9, 10, 12, 16, 30, 33, 34), male 'Non-Rescue' mice displayed an early lethality, with a median survival age of 67 days (Fig. 2A).
