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Animals that complemented for lethality were tested for stress-sensitive paralysis.
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To identify recombined chromosomes the lines derived from individual balanced offspring were tested for lethality in trans to apUGO35 and via PCR for the lms deletion.
Table 2 lists the studies in which cyanobacterial LPS were tested for lethality in mice.
At the other extreme, we are more confident of those BPMs where, for example, many pairs of genes within each pathway were tested for synthetic lethality.
For instance in a substantial recent survey of double deletions only 0.02% of all possible double deletions were tested for synthetic lethality [ 33].
A total of 5,657 lines were tested for potential lethality or sterility by a first round of single-pair matings (left half of table; see Results and Methods for details).
For these experiments, we went through each of the associated papers and uncovered exactly which pairs of genes were tested for synthetic-lethality relationships.
Mutant alleles of the 45 candidate genes were tested for their ability to suppress the OK107 > unf-induced lethality.
The degree to which our results were influenced by genetic variation specific to the embryonic lethality phenotype remains to be tested by examination of additional embryonic RNAi phenotypes.
A subset of the viable insertion lines, those producing fewer homozygotes than expected, was tested for semi-lethality.
Cerumen from Brazilian stingless bees was tested with the Artemia salina lethality assay and, while some samples presented surprisingly high cytotoxicity, others could be considered non-toxic [39].
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