Additionally, increased embryonal and perinatal lethality was reported for UBE3B-/ mice [ 5].
Although minor embryo lethality was reported, most Cux2 neo/neo mutant mice were born viable and fertile and appeared grossly normal.
In the Netherlands, 12.9% lethality was reported for C. difficile PCR ribotype 027 as compared with 7.0% in other C. difficile PCR ribotypes non-027 (21 ).
The germline-expressed genes were chosen following exploratory examination of a larger set of embryonic genes for which observations of embryonic lethality phenotypes were reported on wormbase.org.org
A top1Δ strain was also analyzed, as top1 trf4 double mutants were reported to show synthetic lethality and rDNA condensation phenotypes (Sadoff et al, 1995; Castano et al, 1996).
In another recent study, mice vaccinated with A/Indonesia/05 VLPs were reported to be protected against lethality after heterologous challenge with an A/Viet Nam H5N1 reassortant virus [31].
The other three candidates were reported to have a range of embryonic lethality by RNAi (tofu-6, C56C10.10, and T14B4.2 ).
Of the 10 candidates, four were reported to have some degree of embryonic lethality by RNAi (rab-10, ekl-1, smgl-1, and chaf-1 ).
In order to better understand the regulatory mechanism as well as the functional effect of MAP, we investigated the methylation status of these genes in mice, for which embryonic lethality has been reported in Wnt2 and Ephb4 knock-outs [28], [29].
However, shRNA delivered by gene therapy vectors has raised concerns because severe toxicity and lethality have been reported in mice (Grimm et al, 2006).
