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Thus a total of 45 suppressors of BRCA1 lethality were identified (Table S1).
Cell death has been successfully employed as an end-point for screening a cDNA library in search of protein inhibitors of Caspases, where cDNA clones that rescued yeast from a Caspase-mediated lethality were identified [8].
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As defects in this gene are known to cause male lethality, surviving female patients were identified most of time.
Complete deletion of Uch37 resulted in prenatal lethality, since no homozygous neonates were identified among 64 pups produced by 10 litters of Uch37+/− mice.
Putative dlg5 mutants were identified by lethality.
Twenty-four UAS insertions were identified that cause lethality when expressed in either the mesoderm (Twi-Gal4) or the ectoderm (69B-Gal4).
Twenty-four insertions were identified that caused lethality upon ectopic expression in the mesoderm and/or ectoderm (Table 1).
In case of lethality, larvae of the right genotype were identified by insc-Gal4-driven CD8::GFP (brand and salivary gland) using a fluorescence microscope.
Three mutations that confer suppression of spt10 Δ poor growth were in two genes and five mutations that cause lethality when combined with spt10 Δ were identified in four genes.
Interactions annotated "Negative Genetic," "Synthetic Growth Defect," or "Synthetic Lethality" were included in this list if both genes were identified as nonessential in our data.
Deletion mutants or deletion mutant plasmid combinations that resulted in synthetic lethality (SL), slow growth (SG), or improved growth (suppressor) were identified (see Materials and Methods).
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