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Per cent lethality was noted [ 12].
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In cases where a knockout model displayed lethality, this was noted separately.
The number of clonic, tonic seizures, and lethality as well as the number of deaths was noted.
Results of the lethality test were noted in terms of percentage of mortality of brine shrimp nauplii.
To be noted, lethality in the CD-knockdown population showing an apparent 'quasi-normal' phenotype reached the maximum at a time in which CD MPO lost its effectiveness.
While it is not possible to definitively attribute the early embryonic lethality observed here to defects in secretion, it should be noted that the yeast homolog of Snap23/Snap25 (SEC9) is essential for cell viability [28].
The correlation between degree and essentiality, in that proteins having more interactions are more likely to be essential, has been noted previously [20] [23] and is generally an accepted precept known as the centrality-lethality rule.
It should be noted that this treatment causes an inactivation of metabolic activity of E. coli rather than a complete lethality.
It should be noted that the above groupings of behaviours are strictly within the dimension of method and no inference should be made about intent, outcome and lethality.
The very small fraction of essential synthetic lethal gene pairs (< 100 synthetic lethalities out of >500,000 possibilities - assuming approx. 1000 non-essential metabolic genes) emphasizes the robustness of S. Typhimurium's metabolic network, which has previously been noted [ 5].
As has been noted, the Dobzhansky-Muller model does not require that the epistatic interaction cause absolute loss of hybrid fitness (i.e., lethality or sterility) (Coyne and Orr 2004).
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