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Physiological parameters were taken from experimental studies where lethality was defined in terms of 50% mortality on sample populations of acclimated birds.
Lethality was defined as cessation of observed movement and respiration.
In addition, lethality was defined as any decrease in cell number in treated wells versus the baseline number of cells pre-treatment at day 1 of exposure.
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Global lethality is defined as the ratio of deaths of a particular cancer over all cancers.
Local lethality is defined as the ratio of deaths of a particular cancer over the cases of that particular cancer.
Then the lethality is defined as 1 - κ, which takes a value between 0 and 1.
Lethality is defined as the lack of homozygous animals upon genotyping (usually near wean); at least 28 animals must be genotyped prior to calling a strain lethal at 95% confidence.
Synthetic lethality is defined as cell death that results from complete inactivation of two genes in cells where inactivation of either alone does not result in death [ 4, 5].
The high-lethality group was defined using the following criteria: 1) mechanical ventilation was required for life support; 2) surgery was performed under general anesthesia; 3) the method of attempted suicide carried a high risk of death, specifically, hanging, gunshot, jumping from a high place, inhalation of gas, solvents, or other agricultural chemicals, thermal injury, or drowning.
The lethality in these selection experiments was defined as the level of oxygen in which D. melanogaster cannot complete development and reproduce.
Here, an essential gene was defined as a gene whose KO phenotype is annotated as lethality (including embryonic, prenatal, and postnatal lethality) or infertility [ 9].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com