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Among the new opa variants, we discovered the rare short opa23 allele that causes embryonic lethality in many backgrounds.
Gastric cancer (GC) is the second leading cause of oncologic death worldwide [ 1, 2], due to its elevated incidence and lethality; in many countries survival at five years of diagnosis is under 20% [ 3].
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Unfortunately, knowledge regarding the role of NFκB during late embryonic development in general, and lung development specifically, has been limited by the embryonic and perinatal lethality observed in many of murine models containing deletions of NFκB family members (Table 1).
Cybrid embryonic lethality has been observed in many distant nucleocytoplasmic combinations.
The next alarming finding with respect to RNAi therapeutic applications came from studies using Pol III expressed shRNAs delivered in an AAV delivered by tail vein injection into mice, which resulted in lethality due to acute liver failure in many of the animals (Grimm et al, 2006).
Indeed, they documented that simultaneous 5-FU addition or administration 2 days after cryotherapy resulted in synergistic lethality, although many cells survived these treatments.
While inactivation of STAT3 leads to embryonic lethality in mice, its function is dispensable in many adult tissues.
ECO1/CTF1 is an essential gene in many organisms with the complete inactivation of CTF7 resulting in lethality [ 1- 3, 14- 16, 21].
Without induction, DUX4 RNA was expressed at low levels in many tissues and animals displayed a variety of unexpected dominant leaky phenotypes, including male-specific lethality.
Water quality in many European rivers improved significantly over the last 30 years, and river waters exhibiting acute lethality to fish or invertebrates are an exception today.
The reduction in viability or lethality seen when many genes are present in only one copy may be due to several factors.
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